Single Cell Diagnostics: Methods and Protocols (Methods in Molecular Medicine)
By addebook • Jun 7th, 2009 • Category: Medicine
Single Cell Diagnostics: Methods and Protocols (Methods in Molecular Medicine)
by: Alan Thornhill
en
1588295788 9781588295781 9781597452984
Single Cell Diagnostics: Methods and Protocols (Methods in Molecular Medicine)
By Alan Thornhill
Publisher: Humana Press
Number Of Pages: 182
Publication Date: 2007-06-15
ISBN-10 / ASIN: 1588295788
ISBN-13 / EAN: 9781588295781
Binding: Hardcover
Single Cell Diagnostics: Methods and Protocols applies modern
molecular diagnostic techniques to the analysis of single cells, small
numbers of cells, or cell extracts. Emphasis is placed on non-invasive
analysis of single cell metabolites and the direct analysis of RNA and
DNA from single cells, with a focus on polymerase chain reaction and
fluorescence in situ hybridization. The authors, all leading
scientists in the field, provide current and innovative methods for
extracting useful, accurate, and reliable data from single-cell
samples. The stepwise, technically precise protocols presented in this
volume thoroughly describe single-cell assays, from material collection
and isolation of cells to data interpretation. Cell-types analyzed
include embryonic blastomeres, fibroblasts, and lymphocytes. Among the
techniques described are real-time quantitative PCR, isothermal whole
genome amplification, comparative genomic hybridization, real-time
genetic expression analysis, and the production of RNA and cDNA
libraries. This handbook will be essential for researchers and
clinicians, and practitioners providing care for couples seeking
treatment for infertility or preimplantation genetic diagnosis.
Please Login or Register to read the rest of this content.
Random Posts
Leave a Reply
You must be logged in to post a comment.
