In Situ Hybridization Protocols 3rd edition (Methods in Molecular Biology 326)
By addebook • Jun 22nd, 2008 • Category: Biology •
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In Situ Hybridization Protocols 3rd edition (Methods in Molecular Biology 326)
Publisher: Humana Press
Number Of Pages: 280
Publication Date: 2005-12-15
Sales Rank: 1019745
ISBN / ASIN: 1588294021
EAN: 9781588294029
Binding: Hardcover
Manufacturer: Humana Press
Studio: Humana Press
Average Rating: 5
This revised and updated edition of a recognized classic emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Review:
THE book for in situ methodologies
With the rapid and on-going development of In Situ hybridisation, a new edition of this book was needed. In Professor Darby the publishers found a very capable editor. He has gathered articles which, while conveying all the protocols you would need to reproduce the techniques in your own laboratory, are surprisingly readable.
Even for the experienced practitioner, there are methods in here that will catch you by surprise. The wealth of illustrations add to the enjoyment, as you gain a very clear idea of what to expect. It is a shame, however, that most are in black and white. The supplement of colour photos (and the page in the professor’s own article) demonstrate the results most clearly.
As the summary says, if you are interested in hybridisation - whether of protein, chromosomes or even whole embryos - this is an indispensable manual.
(caveat: probably not a must-by for the general reader)
http://rapidshare.com/files/39884460/Darb_ISHP3eMMB326.rar
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